منابع مشابه
Serotonin synthesis and release in brain slices: independence of tryptophan.
The purpose of the present study was to examine the role of substrate availability in the regulation of the release and synthesis of serotonin by brain slices. Electrical field depolarization of the brain slices stimulated the synthesis and release of serotonin in the absence of changes in intracellular tryptophan concentration, in the absence of tryptophan in the incubation bath, and in the ab...
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Protein synthesis in brain slices was followed by the incorporation of 14C-amino acid, mostly r4C-valine; it remained steady for at least 4 hours at 37”. When applied for 15 min or longer, electrical stimulation of slices, by use of alternating line current reduced to 0.5 to 3 volts, inhibited protein synthesis increasingly, the inhibition reaching a plateau at 1.5 volts. For comparison, the pa...
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Protein synthesis was studied in slices from rat cerebral cortex and localized in various purified subcellular membrane fractions isolated after incubation with L-leucine. A synaptosomal fraction isolated from a several times washed crude mitochondrial pellet showed very little contamination by free membranes, and the synaptic membrane fraction isolated from it was estimated to c +ain about 50%...
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LiCI-extraction of RNAs from isolated ribosomes, however, destroys the coding properties of mRNAs (as has been shown with cerebral mRNA; P. MANDEL, pers. comm.). Therefore separation of mRNAs from ribosomes was achieved by EDTA. If isolated ribosomes from intact potato tubers are dissociated into subunits and RNAs by EDTA, sucrose density gradient profiles show the presence of the 60Sand 40S-su...
متن کاملPreparation of Brain Slices
Frontiers in Neural Circuits www.frontiersin.org May 2010 | Volume 4 | Article 12 | 1 clature for morphological, physiological and molecular features was agreed upon by the Petilla Interneuron Nomenclature Group (Ascoli et al., 2008). Among GABAergic interneurons, those expressing the neuropeptide somatostatin (SOM) are particularly heterogeneous in their molecular, morphological and electrophy...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96256-3